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picture1_Light Microscopy Pdf 89045 | Instru 4


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File: Light Microscopy Pdf 89045 | Instru 4
unit 1 part 2 microscopy instrumentation and biotechniques dse 7 sem vi dark field microscopy the field surrounding a specimen appears black while the object itself is brightly illuminated background ...

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        Unit 1: part 2 
         Microscopy 
    Instrumentation and Biotechniques, 
         DSE-7, Sem VI 
             
                    Dark field Microscopy 
     •  The field surrounding a specimen appears black, while the 
        object itself is brightly illuminated.  
     •  Background is dark, so this type of microscopy is called dark-
        field microscopy 
     •  used to identify bacteria like the thin and distinctively shaped 
        Treponema pallidum, the causative agent of syphilis 
     •  Living,  unstained  cells  and  organisms  can  be  observed  by 
        simply changing the way in which they are illuminated. 
     •  A hollow cone of light is focused on the specimen in such a 
        way that unreflected and unrefracted rays do not enter the 
        objective. Only light that has been reflected or refracted by 
        the specimen forms an image 
         
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...Unit part microscopy instrumentation and biotechniques dse sem vi dark field the surrounding a specimen appears black while object itself is brightly illuminated background so this type of called used to identify bacteria like thin distinctively shaped treponema pallidum causative agent syphilis living unstained cells organisms can be observed by simply changing way in which they are hollow cone light focused on such that unreflected unrefracted rays do not enter objective only has been reflected or refracted forms an image...

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