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picture1_Learning Methods Pdf 92894 | 07 Estimation  Of Glucose By God Pod 18 12 2018


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File: Learning Methods Pdf 92894 | 07 Estimation Of Glucose By God Pod 18 12 2018
estimation of plasma glucose by god pod method learning objectives 1 principle 2 reaction 3 reagents 4 procedure 5 data 6 calculation 7 result 8 reference range 9 interpretation 10 ...

icon picture PDF Filetype PDF | Posted on 17 Sep 2022 | 3 years ago
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    ESTIMATION OF PLASMA 
  GLUCOSE BY GOD -POD METHOD  
              Learning Objectives 
    1. Principle 
    2. Reaction 
    3. Reagents 
    4. Procedure 
    5. Data     
    6. Calculation 
    7. Result 
    8. Reference Range 
    9. Interpretation 
    10. Other Methods 
        1. Principal 
  • Glucose oxidase (GOD) oxidizes the specific 
   substrate β-D- glucose to gluconic acid and 
   hydrogen peroxide (H2O2) is liberated. 
   Peroxidase (POD) enzyme acts on hydrogen 
   peroxide to liberate nascent oxygen (O2), then 
   nascent oxygen couples with 4- amino 
   antipyrine and phenol to form red 
   quinoneimine dye. 
  • The intensity of the colour is directly 
   proportional to the concentration of glucose 
   present in plasma. The intensity of colour is 
   measured by colorimeter at 530 nm or green 
   filter and compared with that of a standard 
   treated similarly. Final colour is stable for at 
   least 2 hours if not exposed to direct sunlight.  
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...Estimation of plasma glucose by god pod method learning objectives principle reaction reagents procedure data calculation result reference range interpretation other methods principal oxidase oxidizes the specific substrate d to gluconic acid and hydrogen peroxide ho is liberated peroxidase enzyme acts on liberate nascent oxygen o then couples with amino antipyrine phenol form red quinoneimine dye intensity colour directly proportional concentration present in measured colorimeter at nm or green filter compared that a standard treated similarly final stable for least hours if not exposed direct sunlight...

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