Contents 
          • Introduction
          • Preparation of Extraction columns
          • Results
          • Conclusion
                     HYDROMETALLURGY SEMINAR, MARCH 2015
                            Introduction 
     Extraction chromatography:
     • Combination:
        – Selectivity of liquid-liquid extraction
        – Multistage character & rapidity of chromatographic processes
     • Extractant is adsorbed on the surface of an inert support 
     • Separation of the metals is based on the distribution of the 
        cations of interest between an organic and an aqueous phase 
        Include the possibility to use mineral acid as mobile phase
       Amount of organic compound (ligand) required in extraction 
                    chromatography is significantly low
 http://www.triskem-international.com/full_extraction_chromatographie.asp
                          HYDROMETALLURGY SEMINAR, MARCH 2015
                                                   Introduction 
         General parameters of Stationary phases:
         •    Physical stability
               – Selection of suitable support
               – Eluent parameters
         •    Chemical stability
               – Composition of stationary phases change
                      •  Light, temperature & nuclear radiation
               – Possible unwanted redox reactions with stationary phases
                  TBP strongly hydrolyses in the column, when column left as such after using 
                                                                 conc. acids
         •    Regeneration of stationary phases
         •    Repeatability & reproducibility
                                                HYDROMETALLURGY SEMINAR, MARCH 2015
                                  Preparation of columns
                       Extractants 
                      dissolved in 
                     Methanol:water
                         (55:45)
                       Solution is                  Columns                    Column is 
                        passed                    washed with                  ready for 
                        through                   0.1 M HNO                    separation
                        columns                               3
                                                                             Metals can be                  Separated 
                                                                               separated                      metals 
                                                                             using diluted                  detected by 
                                                                              minerls acid                  ICP-MS or 
                                                                            under gradient                 UV/Vis after 
                                                                              or isocratic                     PCR
                                                                                elution
                                                HYDROMETALLURGY SEMINAR, MARCH 2015
                                 Separation Principle
                                                                                             Mobile phase
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                                        HYDROMETALLURGY SEMINAR, MARCH 2015